RNAlater® is a viable storage option for avian influenza sampling in logistically challenging conditions

Authors:

• Wille, Michelle
• Yin, Hong
• Lundkvist, Åke
• Xu, Juan
• Muradrasoli, Shaman
• Järhult, Josef D.

Details:

Journal of Virological Methods, Volume 252, 2018-02-28

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Surveillance of wild birds is critical in monitoring for highly pathogenic avian influenza A viruses (AIVs). However, a successful surveillance regime requires proper treatment of samples in the field – rapid placement of samples in −80°C and subsequent maintenance of cold-chain. Given the logistical difficulties of this, many avian taxa and/or geographic locations are not sampled, or, when sampled may result in false negatives due to poor sample treatment in the field. Here, we assessed the utility of RNAlater® as a stabilization agent for AIV sampling. We found no difference in real time PCR performance between virus transport media at optimal conditions and RNAlater® at −80°C, −20°C, 4°C or room temperature up to two weeks, at either low or high virus load. Not only was RNAlater® useful in comparison of spiked samples or those from duck experiments, it was employed successfully in a field study of backyard birds in China. We detected AIV in cloacal and oropharyngeal samples from chickens and a sample with a low Cq was successfully subtyped as H9, although sample storage conditions were suboptimal. Thus, despite limitations in downstream characterization such virus isolation and typing, RNAlater® is a viable option for AIV sampling under logistically challenging circumstances.