Protocol for production and expression of chimeric bovine-human monoclonal antibodies

Authors:

• Heydarchi, Behnaz
• Purcell, Damian F.J.

Details:

STAR Protocols, Volume 3, Issue 4, 2022-12-16

Article Link: Click here

We describe herein a protocol for production of chimeric bovine-human monoclonal antibodies (mAbs) from vaccinated cows. The genes of HIV-1-specific single B cells are amplified by reverse transcription-polymerase chain reaction (RT-PCR), cloned into human expression vectors, and expressed in human cell lines. This protocol provides an efficient step-by-step methodology to produce HIV-1 chimeric mAbs and could be widely adapted for other antigens. For complete details on the use and execution of this protocol, please refer to Heydarchi et al. (2022). 1